Title A physical and genetic map of Cannabis sativa identifies extensive rearrangement at the THC/CBD acid synthase locus
Author Laverty KU, Stout JM, Sullivan MJ, Shah H, Gill N, Holbrook L, Deikus G, Sebra R, Hughes TR, Page JE, Bakel H van
Issue Genome Res gr.242594.118
Abstract Cannabis sativa is widely cultivated for medicinal, food, industrial, and recreational use, but much remains unknown regarding its genetics, including the molecular determinants of cannabinoid content. Here, we describe a combined physical and genetic map derived from a cross between the drug-type strain ‘Purple Kush’ and the hemp variety ‘Finola’. The map reveals that cannabinoid biosynthesis genes are generally unlinked, but that aromatic prenyltransferase (AP), which produces the substrate for THCA and CBDA synthases (THCAS and CBDAS), is tightly linked to a known marker for total cannabinoid content. We further identify the gene encoding CBCA synthase (CBCAS) and characterize its catalytic activity, providing insight into how cannabinoid diversity arises in cannabis. Strikingly, THCAS and CBDAS (which determine the drug vs hemp chemotype) are contained within large (>250 kb) retrotransposon-rich regions that are highly non-homologous between drug- and hemp-type alleles, and are furthermore embedded within ~40 Mb of non-recombining repetitive DNA. The chromosome structures are similar to those in grains such as wheat, with recombination focused in gene-rich, repeat-depleted regions near chromosome ends. The physical and genetic map should facilitate further dissection of genetic and molecular mechanisms in this commercially and medically important plant.
Link http://genome.cshlp.org:4040/lookup/pmidlookup?view=long&pmid=30409771 



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